聚谷氨酸苄酯（PBLG）,　聚多肽

聚谷氨酸苄酯（PBLG）,　聚多肽

一、产品规格：

二、产品介绍：

三、使用方法：

（一）玻片涂膜（用于细胞培养）

1、取一定量聚合物溶于氯仿，制成0.1%聚合物溶液。为获得均质薄膜，溶解过程中需搅拌子搅拌或超声处理20－30min，以确保聚合物完全溶解。

2、取10－30ul聚合物溶液，滴加在硅化玻片表面，快速推片或拉片涂膜，常温下自然干燥30min，真空干燥6－12h。

3、置于多孔板中，紫外照射10－15分钟，或臭氧熏蒸30分钟后用于细胞培养。

4、根据研究目的不同，还可对玻片表面的聚合物薄膜进行表面修饰处理。不同组别可设置3－5个平行样，以便进行定量分析。

5、其它研究方法可参照文献进行。

（二）使用方法及案例（参考文献）：

1、骨组织工程支架

① 溶液浇铸/粒子沥滤法

In vivo mineralization and osteogenesis of nanocomposite scaffoldof poly(lactide-co-glycolide) and hydroxyapatite surface-grafted withpoly(l-lactide). Biomaterials, 2009, 30:58-70.
Peibiao Zhang, Zhongkui Hong, Ting Yu, Xuesi Chen, Xiabin Jing.

RGD-conjugated copolymer incorporated into composite ofpoly(lactide-co-glycotide) and poly(l-lactide)-grafted nanohydroxyapatite forbone tissue engineering. Biomacromolecules, 2011, 12(7):2667-2680.
PeibiaoZhang, Haitao Wu, Han Wu, ZhongwenLu, Chao Deng, Zhongkui Hong,XiabinJing, XuesiChen

In vivo degradation behavior of porous composite scaffolds ofpoly(lactide-co-glycolide) and nano-hydroxyapatite surface grafted withpoly(L-lactide). Chinese Journal of Polymer Science, 2014, 32(6):805-816.
Yu-feng Tang, Jian-guo Liu, Zong-liang Wang, Yu Wang, Li-guo Cui, Pei-biaoZhang*, Xue-si Chen.

Tissue-engineered composite scaffold ofpoly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded withautologous mesenchymal stem cell (MSCs) for bone regeneration. J ZhejiangUniv-Sci B (Biomed & Biotechnol), 2017, 18(11):963-976.
Bing ZHANG, Pei-biao ZHANG, Zong-liang WANG, Han WU.

② 熔融成型/粒子沥滤法

The Nano-Composite Scaffold of Poly(lactide-co-glycolide)andHydroxyapatite Surface-Grafted with L-lactic Acid Oligomer for Bone Repair.Acta Biomaterialia, 2009, 5:2680-2692.
Yang Cui, Yi Liu, Yi Cui, Xiabin Jing, Peibiao Zhang*, XuesiChen.

Enhanced in vitro mineralization and in vivo osteogenesis ofcomposite scaffolds through controlled surface grafting of L-lactic acidoligomer on nano-hydroxyapatite. Biomacromolecules, 2016, 17(3):818-829.
Wang, Zongliang, Xu, Yang, Wang, Yu , Ito, Yoshihiro, Zhang, Peibiao*, Chen,Xuesi

③ 单溶剂冷冻成型法

Preparation ofPorous Nanocomposite Scaffolds with HoneycombMonolith Structure by OnePhase Solution Freeze-Drying Method. Chinese Journalof Polymer Science, 2011, 29(2):215-224.
Yang Xu, Duo Zhang**, Zong-liang Wang, Zhan-tuanGao, Pei-biao Zhang* and Xue-siChen.

④ 可注射原位固化成型法

An injectable hydroxyapatite/poly(lactide-co-glycolide)composite reinforced by micro/nano-hybrid poly(glycolide) fibers for bonerepair. Materials Science and Engineering: C, 2017, 80:326-334.
Yuhang Zhu,Zongliang Wang,Hongli Zhou,Linlong Lib,Qingsan Zhu*,Peibiao Zhang*.

In vitro degradation behavior ofhydroxyapatite/poly(lactide-co-glycolide) composite reinforced bymicro/nano-hybrid poly(glycolide) fibers for bone repair. Journal of MaterialsChemistry B, 2017, 5(44):8695-8706.
Yuhang Zhu, Zongliang Wang*, Linlong Li, Daqian Gao, Qinli Xu, Qingsan Zhu*,Peibiao Zhang*.

⑤ 静电纺丝载药纤维毡

Methylsulfonylmethane-loaded electrospunpoly(lactide-coglycolide) mats for cartilage tissue engineering. RSC Advances,2015, 5:96725-32.
Zongliang Wang, Yu Wang, Peibiao Zhang*, and Xuesi Chen.

⑥ 熔融旋碟纺丝纤维

A Novel Nano/Micro-Fibrous Scaffold by Melt-Spinning Method forBone Tissue Engineering. Journal of Bionic Engineering, 2015, 12(1):117-128.
Liguo Cui, Ning Zhang, Weiwei Cui, Peibiao Zhang, Xuesi Chen.

Improved cellular infiltration into 3D interconnectedmicrochannel scaffolds formed by using melt-spun sacrificial microfibers. RSCAdvances, 2016, 6:2131-33.
Zongliang Wang, Tianlin Gao, Liguo Cui, Yu Wang, Peibiao Zhang* and Xuesi Chen.

⑦ 溶剂交换3D打印成型法

3D-printing of solvent exchange deposition modeling (SEDM) for abilayered flexible skin substitute of poly (lactide-co-glycolide) withbioorthogonally engineered EGF. Materials Science & Engineering C, 2020,112:110942.
Daqian Gao, Zongliang Wang, Zhenxu Wu, Xincui Shi, Min Guo, Yu Wang*, ZhenhuaGao*, and Peibiao Zhang*, Yoshihiro Ito.

⑧ 微流控二流法制备微载体(微球)

Assessment of nano-Hydroxyapatite and Poly(Lactide-co-Glycolide) nanocomposite microspheres fabricated by novel airflowshearing technique for in vivo bone repair.Materials Science and Engineering:C,2021, 128: 112299
Song Wenzhi*, Wang Dezhou, Guo Min, Han Chunyu, Zhao Lanlan, Zhang Peibiao*.

2、涂膜法研究成骨活性

聚乳酸接枝改性纳米生物玻璃/PLGA复合材料的制备、表面性质及生物活性. 高等学校化学学, 2009, 30(5):1018-1023.
董树君, 于婷, 魏俊超, 景遐斌, 周延民, 章培标*, 陈学思.

改性纳米羟基磷灰石/PLGA复合材料的制备及生物活性. 高等学校化学学, 2009, 30(7):1439-1444.
于婷, 刘娅, 王宇, 景遐斌, 章培标*, 陈学思.

脉冲电刺激对改性纳米羟基磷灰石/聚丙交酯-乙交酯复合材料表面成骨细胞增殖及成骨活性的影响[J]. 中国组织工程研究与临床康复, 2009, 13(16):3065-3069.
于婷, 刘娅, 邬海涛, 崔巍巍, 王宇, 景遐斌, 章培标*, 陈学思.

电活性可生物降解纳米复合材料PAP/op-HA/PLGA的制备及成骨活性. 高等学校化学学, 2011, 32(5):1181-1187.
邬海涛, 于婷, 朱庆三, 焦自学, 危岩, 章培标*, 陈学思.

Photo-immobilization of Bone Morphogenic Protein 2 on PLGA/HANanocomposite to Enhance the Osteogenesis of Adipose-Derived Stem Cells. RSCAdv., 2016, 6:20202-20210.
Tianlin Gao, Weiwei Cui, Zongliang Wang, Yu Wang, Ya Liu, Ponnurengam SivakumarMalliappan, Yoshihiro Ito and Peibiao Zhang*.

Improved Cell Adhesion and Osteogenesis of op-HA/PLGA Compositeby Poly(dopamine)-Assisted Immobilization of Collagen Mimetic Peptide and OsteogenicGrowth Peptide. ACS Applied Materials & Interfaces, 2016, 8(40):26559-69.
Wang, Zongliang; Chen, Li; Wang, Yu; Chen, Xuesi; Zhang, Peibiao*.

3、功能材料开发

① 载IGF-1微载体用于骨再生

Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) andNano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for EnhancingCell Proliferation and Osteogenic Differentiation. Macromol Biosci., 2015,15(8):1070-80.
Tianlin Gao, Ning Zhang, Zongliang Wang, Yu Wang, Ya Liu, Yoshihiro Ito,Peibiao Zhang*.

DOPA-derived electroactive copolymer and IGF-1 immobilizedpoly(lactic-co-glycolic acid)/hydroxyapatite biodegradable microspheres forsynergistic bone repair.Chemical Engineering Journal, 2021, 416: 129129
Huanhuan Yan, Zongliang Wang, Linlong Li, Xincui Shi, Erna Jia, Qingming Ji, YuWang*, Yoshihiro Ito, Yen Wei, Peibiao Zhang*.

② 可MRI示踪材料

In vivo MRI and X-ray bifunctional imaging of polymericcomposite supplemented with GdPO4*H2O nanobundles for tracing bone implant andbone regeneration. Advanced Healthcare Materials, 2016, 5(17):2182-90.
Jing Huang; Zhongwen Lv; Yu Wang; Zongliang Wang; Tianlin Gao; Ning Zhang; MinGuo; Haifeng Zou*; Peibiao Zhang*.

Incorporation of Gadolinium Oxide and Gadolinium OxysulfideMicrospheres: MRI/CT Monitoring and Promotion of Osteogenic/ChondrogenicDifferentiation for Bone Implants.ChemNanoMat,2020,6(12):1819-1832.
Jing Huang, Zhaoyun Tang, Min Guo, Yu Wang, Zongliang Wang, Zhenxu Wu, PeibiaoZhang*.

Biodegradable GdPO4·H2O/PLGAmicrocarriers for stem cell delivery and non-invasive MRI translocationtracing[J]. Journal of Materials Science, 2022, 57(28):13632-13646.
Wenhao Zhu，Zhenxu Wu，Peng Wang，Tianyi Liu，Min Guo，JiansongJi，Mingfeng He*，Haifeng Wang，Peibiao Zhang*

③ 载DOPA-IGF-1用于神经再生

A Novel Approach via Surface Modification of Degradable PolymersWith Adhesive DOPA-IGF-1 for Neural Tissue Engineering. J Pharm Sci., 2019,108(1):551-562.
Yi Zhang, Zongliang Wang, Yu Wang, Linlong Li, Zhenxu Wu, Yoshihiro Ito, XiaoyuYang*, Peibiao Zhang

Nerve implants with bioactive interfaces enhance neuriteoutgrowth and nerve regeneration in vivo. Colloids and Surfaces B:Biointerfaces, 2022, 218: 112731.
Zongliang Wang, Yi Zhang, Liqiang Wang, Yoshihiro Ito, Gang Li, Peibiao Zhang*.

④ DOPA-NGF微载体用于神经再生

Bioorthogonal DOPA-NGF activated tissue engineering microunitsfor recovery from traumatic brain injury by microenvironment regulation. ActaBiomaterialia, 2022, 150: 67-82.
Wenhao Zhu, Li Chen, Zhenxu Wu, Wenzhong Li, Xiaolong Liu, Yu Wang, Min Guo,Yoshihiro Ito, Liqiang Wang, Peibiao Zhang*, Haifeng Wang*.

⑤ 电活性导电复合材料

电活性和生物活性多巴-胰岛素样生长因子-1@聚(乙交酯-丙交酯)/聚(3-己基噻吩)静电纺丝纤维的制备及神经组织工程应用.应用化学,2019, 36 (9): 1003-1014.
张守燕, 胡江磊, 史新翠, 章培标, 伊藤嘉浩

Electroactive Composite of FeCl3 -Doped P3HT/PLGA withAdjustable Electrical Conductivity for Potential Application in Neural TissueEngineering.Macromol Biosci. 2019,19(10):e1900147.
Shouyan Zhang, Huanhuan Yan, Jui‐Ming Yeh, Xincui Shi*,Peibiao Zhang*.

⑥ 磁响应性智能材料

Synergistic osteogenesis promoted by magnetically actuatednano-mechanical stimuli. Nanoscale, 2019, 11, 23423-2343.
Hao, Lili; Li, Linlong; Wang, Peng; Wang, Zongliang; Shi, Xincui; Guo, Min ;Zhang, Peibiao*.

Spatiotemporal magnetocaloric microenvironment for guiding thefate of biodegradable polymer implants. Advanced Functional Materials, 2021,31(15): 2009661.
L. Hao, J. Li, P. Wang, Z. Wang, Z. Wu,Y. Wang, Z. Jiao, M. Guo,* T. Shi,Q.Wang, Y. Ito, Y. Wei,P. Zhang*.

⑦ 电和磁共响应性智能材料

The electric and magnetic responsive nanocomposite of GdPO4·H2O/P3HT/PLGA with electrical stimulation for synergisticallyenhancing the proliferation and differentiation of pre-osteoblast.New Journalof Chemistry,2019,43:17315-17326
Yan, Huanhuan; Li, Linlong; Wang, Yu; Huang, Jing; Wang, Zongliang; Shi,Xincui; Zhang, Peibiao*.

⑧ 可载干细胞PVA /PLGA双层敷料

Stem Cell Seeded and Silver Nanoparticles Loaded BilayerPLGA/PVA Dressings for Wound Healing.Macromolecular Bioscience,2020,20(10):e2000141
Tianlin Gao, Chunyan Tian, Zepeng Ma, Zunyan Chu, Zongliang Wang, PeibiaoZhang.

⑨ 压电性双示踪智能材料

Gadolinium-doped BTO Functionalized Nanocomposites with EnhancedDual-imaging of MRI and X-Ray to Simulate the Electrical Properties of Bone”. ACS Applied Materials & Interfaces, 2020, 12(44): 49464-49479
Peng Wang, Lili Hao, Zongliang Wang, Yu Wang, Min Guo*, Peibiao Zhang

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