Conventional cell culture is carried out under constant gravity. Due to the effect of
gravity, cells naturally settle in the culture medium, which limits the random combination
and spatial positioning between cells and between cells and matrix, and cannot form a
three-dimensional structure well.
In the late 1980s, NASA designed a rotating wall vessel bioreactor (RWVB) during space
biology experiments.
The rotary wall bioreactor consists of two horizontally placed inner and outer centripetal
circular rods (see figure below). The static inner column is composed of a semi
permeable membrane, allowing gas to exchange through the membrane. The rotating
outer column is made of non permeable material. Continuous infusion of culture
medium between inner and outer columns to culture cells. The entire device can rotate
around the inner axis. By adjusting the rotational speed of the outer column, the
centrifugal force generated by rotation is balanced with gravity, providing a
microgravity environment for cell growth. Under normal culture conditions, mammalian
cells that can only grow in a two-dimensional adherent manner exhibit
three-dimensional proliferation and cell differentiation, further forming functional
tissue blocks.
This bioreactor has been used in bone marrow mesenchymal stem cells, calf
chondrocytes, dog chondrocytes, rat cardiomyocytes, rat pecking gland epithelial cells,
follicular tumor cells, and human pancreatic islets β Progress has been made in cell, liver
cell culture, and thymic organ culture.
NASA currently has four unique cultivation systems:
1. A slow rotating single end fixed container or STL is a batch culture container
2. The perfusion culture system originally designed for perfusion culture and adherent
cell culture
3. High cross-sectional aspect ratio or HAR containers originally designed for wall
attachment and suspension culture
4. Control the spatial bioreactor of tissue culture system through complex process
control computers.
All these containers are built on the basis of reducing gravity factors by rotating along
the horizontal axis. These cultivation systems serve as unique research tools by
simulating the microgravity environment in space in the laboratory:
1. Low scissor force
2. Co cultivation of particles of different sizes
3. Helps to form a three-dimensional organization
4. Helps with cell differentiation
[Significant Significance]
1. Tissue transplantation: For example, 3DB cultured liver and natural liver have no
overall difference, making local tissue transplantation possible.
2. Vaccine production: One of the reasons why hepatitis C vaccines were not effective in
the past was that the virus used to produce this vaccine did not grow in the human liver.
Now, 3DB cultured liver allows the virus that produces the hepatitis vaccine to grow in
the human liver, making it a reality. 3DB has been widely used in production in the
United States.
3. Cartilage regeneration: Cultivated cartilage has extremely high density and can treat
joint injuries.
Hormones, enzymes, and other proteins produced by human tissues, as well as genetic
engineering: Cultured highly differentiated human tissues that can secrete therapeutic
proteins upon stimulation. For example, the nerve growth hormone produced by
cultured neural tissue can repair spinal injuries.
4. Bone marrow regeneration: The cultured bone marrow has excellent growth and can
undergo continuous proliferation and low-temperature cryopreservation, making it
possible to build a large bone marrow bank.
5. diabetes: insulin can be inserted into the body and continue to grow after culture, and
countless patients are expected to avoid long-term insulin injection.
6. Effective tumor killing: Take live samples of tumor tissue, mix them with one's own
white blood cells or lymphocytes in 3DB, stimulate or domesticate them to recognize
and attack tumor tissue, and then directly inject the domesticated cells with killing power
into the lesion. In this way, these infected lymphocytes completely killed the tumor.
7. Ideal models for tumors, AIDS, kidney disease, and heart disease: Cultivate human
organs, glands, and lymph nodes. Then infect these organoids and track their growth.
Apply drugs to infected cultured tissue models to study their effectiveness in combating
diseases and their ways of combating them. For example, the cultivation of tumor tissue
is beneficial for testing the efficacy of chemotherapy drugs on the cultured and
differentiated tumors of patients themselves, avoiding the blindness of medication.
Previously, this was done on a mouse tissue model, but species differences resulted in
poor growth of many tumors in mice. In 3DB, all tumor tissues grew intact and avoided
interference from the original mouse protein.
8. Skin transplantation: Cultivated skin has a high degree of differentiation, avoiding the
shortcomings of previously cultivated skin in texture, flexibility, and skin tone.
(See details: http://www.labbase.net/supply/supplyitems-1387109.html ）
